Immunolocalization of hemicelluloses in Arabidopsis thaliana stem. Part II: Mannan deposition is regulated by phase of development and its patterns of temporal and spatial distribution differ between cell types.
Identifieur interne : 002A32 ( Main/Exploration ); précédent : 002A31; suivant : 002A33Immunolocalization of hemicelluloses in Arabidopsis thaliana stem. Part II: Mannan deposition is regulated by phase of development and its patterns of temporal and spatial distribution differ between cell types.
Auteurs : Jong Sik Kim [Suède] ; Geoffrey DanielSource :
- Planta [ 1432-2048 ] ; 2012.
Descripteurs français
- KwdFr :
- Anticorps monoclonaux (MeSH), Immunohistochimie (MeSH), Mannanes (immunologie), Mannanes (métabolisme), Microscopie de fluorescence (MeSH), Paroi cellulaire (métabolisme), Polygalacturonase (composition chimique), Polyosides (analyse), Polyosides (immunologie), Populus (cytologie), Populus (métabolisme), Tiges de plante (composition chimique), Tiges de plante (croissance et développement), Tiges de plante (cytologie), Tiges de plante (métabolisme), Xylanes (métabolisme).
- MESH :
- analyse : Polyosides.
- composition chimique : Polygalacturonase, Tiges de plante.
- croissance et développement : Tiges de plante.
- cytologie : Populus, Tiges de plante.
- immunologie : Mannanes, Polyosides.
- métabolisme : Mannanes, Paroi cellulaire, Populus, Tiges de plante, Xylanes.
- Anticorps monoclonaux, Immunohistochimie, Microscopie de fluorescence.
English descriptors
- KwdEn :
- Antibodies, Monoclonal (MeSH), Cell Wall (metabolism), Immunohistochemistry (MeSH), Mannans (immunology), Mannans (metabolism), Microscopy, Fluorescence (MeSH), Plant Stems (chemistry), Plant Stems (cytology), Plant Stems (growth & development), Plant Stems (metabolism), Polygalacturonase (chemistry), Polysaccharides (analysis), Polysaccharides (immunology), Populus (cytology), Populus (metabolism), Xylans (metabolism).
- MESH :
- chemical , analysis : Polysaccharides.
- chemical , chemistry : Polygalacturonase.
- chemical , immunology : Mannans, Polysaccharides.
- chemical , metabolism : Mannans, Xylans.
- chemical : Antibodies, Monoclonal.
- chemistry : Plant Stems.
- cytology : Plant Stems, Populus.
- growth & development : Plant Stems.
- metabolism : Cell Wall, Plant Stems, Populus.
- Immunohistochemistry, Microscopy, Fluorescence.
Abstract
Microdistribution of mannans in Arabidopsis stem was examined using immunolocalization with mannan-specific monoclonal antibodies (LM21 and LM22). Mannan labeling in secondary xylem cells (except for protoxylem vessels) was initially detected in the cell wall during S(2) formation and increased gradually during development. Labeling in metaxylem vessels (vessels) was detected earlier than that in xylary fibers (fibers), but was much weaker than fibers. The S(1) layer of vessels and fibers showed much less labeling than the S(2) layer. Some strong labeling was also detected in pit membranes of vessel pits. Interfascicular fibers (If-fibers) showed more heterogeneous labeling patterns than fibers by LM21. Unlike fibers, If-fibers also revealed some strong labeling in the cell corner of the S(1) layer, indicating different mannan labeling patterns between If-fibers and fibers. Interestingly, protoxylem vessels (proto-vessels) showed strong labeling at the early stage of secondary xylem formation with more intense labeling in the outer- than inner cell wall even though fibers and vessels showed no or very low labeling at this stage. Labeling intensity of proto-vessels was also much stronger than vessels and stronger or slightly weaker than fibers by LM21 and LM22, respectively. Using pectinase and mild alkali treatment, the presence of mannans in parenchymatous cells was also confirmed. Together our observations indicate that there are temporal and spatial variations in mannan labeling between cell types in the secondary xylem of Arabidopsis stems. Some similar features of mannan labeling between Arabidopsis and poplar are also discussed.
DOI: 10.1007/s00425-012-1687-x
PubMed: 22718312
Affiliations:
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Box 7008, 750 07, Uppsala</wicri:regionArea><wicri:noRegion>Uppsala</wicri:noRegion></affiliation></author><author><name sortKey="Daniel, Geoffrey" sort="Daniel, Geoffrey" uniqKey="Daniel G" first="Geoffrey" last="Daniel">Geoffrey Daniel</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2012">2012</date><idno type="RBID">pubmed:22718312</idno><idno type="pmid">22718312</idno><idno type="doi">10.1007/s00425-012-1687-x</idno><idno type="wicri:Area/Main/Corpus">002993</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">002993</idno><idno type="wicri:Area/Main/Curation">002993</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">002993</idno><idno type="wicri:Area/Main/Exploration">002993</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Immunolocalization of hemicelluloses in Arabidopsis thaliana stem. Part II: Mannan deposition is regulated by phase of development and its patterns of temporal and spatial distribution differ between cell types.</title><author><name sortKey="Kim, Jong Sik" sort="Kim, Jong Sik" uniqKey="Kim J" first="Jong Sik" last="Kim">Jong Sik Kim</name><affiliation wicri:level="1"><nlm:affiliation>Wood Science, Department of Forest Products, Swedish University of Agricultural Sciences, P.O. Box 7008, 750 07, Uppsala, Sweden. jongsik.kim@slu.se</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Wood Science, Department of Forest Products, Swedish University of Agricultural Sciences, P.O. Box 7008, 750 07, Uppsala</wicri:regionArea><wicri:noRegion>Uppsala</wicri:noRegion></affiliation></author><author><name sortKey="Daniel, Geoffrey" sort="Daniel, Geoffrey" uniqKey="Daniel G" first="Geoffrey" last="Daniel">Geoffrey Daniel</name></author></analytic><series><title level="j">Planta</title><idno type="eISSN">1432-2048</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibodies, Monoclonal (MeSH)</term><term>Cell Wall (metabolism)</term><term>Immunohistochemistry (MeSH)</term><term>Mannans (immunology)</term><term>Mannans (metabolism)</term><term>Microscopy, Fluorescence (MeSH)</term><term>Plant Stems (chemistry)</term><term>Plant Stems (cytology)</term><term>Plant Stems (growth & development)</term><term>Plant Stems (metabolism)</term><term>Polygalacturonase (chemistry)</term><term>Polysaccharides (analysis)</term><term>Polysaccharides (immunology)</term><term>Populus (cytology)</term><term>Populus (metabolism)</term><term>Xylans (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Anticorps monoclonaux (MeSH)</term><term>Immunohistochimie (MeSH)</term><term>Mannanes (immunologie)</term><term>Mannanes (métabolisme)</term><term>Microscopie de fluorescence (MeSH)</term><term>Paroi cellulaire (métabolisme)</term><term>Polygalacturonase (composition chimique)</term><term>Polyosides (analyse)</term><term>Polyosides (immunologie)</term><term>Populus (cytologie)</term><term>Populus (métabolisme)</term><term>Tiges de plante (composition chimique)</term><term>Tiges de plante (croissance et développement)</term><term>Tiges de plante (cytologie)</term><term>Tiges de plante (métabolisme)</term><term>Xylanes (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Polysaccharides</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Polygalacturonase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Mannans</term><term>Polysaccharides</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Mannans</term><term>Xylans</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Antibodies, Monoclonal</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Polyosides</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Plant Stems</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Polygalacturonase</term><term>Tiges de plante</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Tiges de plante</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Populus</term><term>Tiges de plante</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Plant Stems</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Plant Stems</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Mannanes</term><term>Polyosides</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Wall</term><term>Plant Stems</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Mannanes</term><term>Paroi cellulaire</term><term>Populus</term><term>Tiges de plante</term><term>Xylanes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Immunohistochemistry</term><term>Microscopy, Fluorescence</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Anticorps monoclonaux</term><term>Immunohistochimie</term><term>Microscopie de fluorescence</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Microdistribution of mannans in Arabidopsis stem was examined using immunolocalization with mannan-specific monoclonal antibodies (LM21 and LM22). Mannan labeling in secondary xylem cells (except for protoxylem vessels) was initially detected in the cell wall during S(2) formation and increased gradually during development. Labeling in metaxylem vessels (vessels) was detected earlier than that in xylary fibers (fibers), but was much weaker than fibers. The S(1) layer of vessels and fibers showed much less labeling than the S(2) layer. Some strong labeling was also detected in pit membranes of vessel pits. Interfascicular fibers (If-fibers) showed more heterogeneous labeling patterns than fibers by LM21. Unlike fibers, If-fibers also revealed some strong labeling in the cell corner of the S(1) layer, indicating different mannan labeling patterns between If-fibers and fibers. Interestingly, protoxylem vessels (proto-vessels) showed strong labeling at the early stage of secondary xylem formation with more intense labeling in the outer- than inner cell wall even though fibers and vessels showed no or very low labeling at this stage. Labeling intensity of proto-vessels was also much stronger than vessels and stronger or slightly weaker than fibers by LM21 and LM22, respectively. Using pectinase and mild alkali treatment, the presence of mannans in parenchymatous cells was also confirmed. Together our observations indicate that there are temporal and spatial variations in mannan labeling between cell types in the secondary xylem of Arabidopsis stems. Some similar features of mannan labeling between Arabidopsis and poplar are also discussed.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">22718312</PMID><DateCompleted><Year>2013</Year><Month>04</Month><Day>25</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1432-2048</ISSN><JournalIssue CitedMedium="Internet"><Volume>236</Volume><Issue>5</Issue><PubDate><Year>2012</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Planta</Title><ISOAbbreviation>Planta</ISOAbbreviation></Journal><ArticleTitle>Immunolocalization of hemicelluloses in Arabidopsis thaliana stem. 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Interfascicular fibers (If-fibers) showed more heterogeneous labeling patterns than fibers by LM21. Unlike fibers, If-fibers also revealed some strong labeling in the cell corner of the S(1) layer, indicating different mannan labeling patterns between If-fibers and fibers. Interestingly, protoxylem vessels (proto-vessels) showed strong labeling at the early stage of secondary xylem formation with more intense labeling in the outer- than inner cell wall even though fibers and vessels showed no or very low labeling at this stage. Labeling intensity of proto-vessels was also much stronger than vessels and stronger or slightly weaker than fibers by LM21 and LM22, respectively. Using pectinase and mild alkali treatment, the presence of mannans in parenchymatous cells was also confirmed. Together our observations indicate that there are temporal and spatial variations in mannan labeling between cell types in the secondary xylem of Arabidopsis stems. Some similar features of mannan labeling between Arabidopsis and poplar are also discussed.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Jong Sik</ForeName><Initials>JS</Initials><AffiliationInfo><Affiliation>Wood Science, Department of Forest Products, Swedish University of Agricultural Sciences, P.O. Box 7008, 750 07, Uppsala, Sweden. jongsik.kim@slu.se</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Daniel</LastName><ForeName>Geoffrey</ForeName><Initials>G</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2012</Year><Month>06</Month><Day>21</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Planta</MedlineTA><NlmUniqueID>1250576</NlmUniqueID><ISSNLinking>0032-0935</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000911">Antibodies, Monoclonal</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D008351">Mannans</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011134">Polysaccharides</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014990">Xylans</NameOfSubstance></Chemical><Chemical><RegistryNumber>8024-50-8</RegistryNumber><NameOfSubstance UI="C007916">hemicellulose</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.15</RegistryNumber><NameOfSubstance UI="D011096">Polygalacturonase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000911" MajorTopicYN="N">Antibodies, Monoclonal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002473" MajorTopicYN="N">Cell Wall</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007150" MajorTopicYN="N">Immunohistochemistry</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008351" MajorTopicYN="N">Mannans</DescriptorName><QualifierName UI="Q000276" 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PubStatus="medline"><Year>2013</Year><Month>4</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">22718312</ArticleId><ArticleId IdType="doi">10.1007/s00425-012-1687-x</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Planta. 2003 Nov;218(1):27-36</Citation><ArticleIdList><ArticleId IdType="pubmed">12844268</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2010 Oct;64(2):191-203</Citation><ArticleIdList><ArticleId IdType="pubmed">20659281</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 1992 Nov;4(11):1413-24</Citation><ArticleIdList><ArticleId IdType="pubmed">1477555</ArticleId></ArticleIdList></Reference><Reference><Citation>Planta. 2010 Jul;232(2):545-54</Citation><ArticleIdList><ArticleId IdType="pubmed">20499086</ArticleId></ArticleIdList></Reference><Reference><Citation>J Agric Food Chem. 2008 Aug 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